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If poor. 1) Decreasing the dissolved air in the eluent increases the refractive index.
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  • We are having a fairly intractable problem with a baseline "ripple" or "wave" effect at certain gradients at 220 nm which is effectively killing our LOQ and LOD (small peaks look indistinguishable from this "noise", causing our noise level to effectively appear higher for s/n calculations).
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    Typically, your pump will have two pistons and seals.

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  • May 25, 2022 · Baseline noise is classified as the difference between the largest and the smallest point of the baseline of a blank run chromatogram.
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    Again, the contamination source should be traced and.

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  • To minimize baseline noise and drift solvents must be LC grade and filtered prior to use.
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    Note the the max and min drift on the y axis (I like to see a maximum delta of only a few milli aus on the Y axis) Now pull out the flow cell and do the same thing for 15 min.

  • Nov 10, 2022 · This week we are going to learn why smoothing the chromatogram can be helpful (Part 7).
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    The formation or accumulation of bubbles in the detector cell can cause spikes and sawtooth noise or cause the baseline to fluctuate out of range.

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    The “Auto Threshold” option measures the peak-to-peak noise of this.